Application

Full-length RNA

Single-cell RNA

16S rRNA

Sequencing goal

Isoform discovery and abundance information

Cell type-specific isoform isoform discovery and abundance information

Species identification in metagenomics samples

Short-read shortcomings

Incorrect isoform discovery and abundance information

No isoformal. Only genes 

No species identification 

Other long-read drawbacks

Lower accuracy  poor transcript identification and variant calling

Lower accuracy  poor transcript identification and variant calling 

Lower accuracy  poor transcript identification and variant calling 

HiFi advantages

Isoform discovery and abundance information 

Cell type-specific isoform discovery 

Species-level identification 

Kinnex full-length RNA kit

Kinnex single-cell RNA kit

Kinnex 16S rRNA kit

Kit advantage 

Full-length isoform discovery with flexible sample multiplexing

Cell type-specific isoform isoform discovery with the highest accuracy

Full-length 16S rRNA for species identification 

Total throughput 

15-20M (Sequel II)

30-40M (Revio)

30-40M (Sequel II)

80-100M (Revio)

20-25M (Sequel II)

50-60M (Revio)

Sample multiplexing recommendation

5M × 3-plex (Sequel II)

10M × 4-plex (Revio)

1-plex (Sequel II)

1- or 2-plex (Revio)

384-plex (Sequel II)

384- to 1,536-plex (Revio)

Analysis 

SMRT Link v13.0 and above 

SMRT Link v13.0 and above 

SMRT Link v13.0 and above