Navinci
Diagnostics
(주)엠디엑스케이는 고객의 연구 목적에
적합한 기술과 서비스를 제공합니다.
-
PRODUCT
- NAVINCI DIAGNOSTICS
Brand
Next-generation immunostaining of cells and tissues
in situ protein analysis.
Navinci Diagnostics는 단백질 간의 interaction 및 단백질의 modification, expression을 확인할 수 있는 Proximity Ligation Assay를 제공합니다.
Technology
Proximity ligation with Navenibodies
-
-
Probe incubationUnfolding Proximity Probe가 결합된 2차 항체 처리
-
- Probe activation
-
- Template formationLigase를 넣어
Circle 형태로 만듦
-
- Signal amplificationrolling circle amplification
-
- Visualization and data processing형광현미경 관찰
Easy sample-to-results workflow
Workflow
Advantage / Features
Advantage
-
Clear target detection
단백질의 과발현없이 현미경으로 직접 발현확인
Higher Signal to Noise ratio : Reduced tissue background
-
Sensitive in situ detection
Endogenous 상태에서 발현량이 약하거나 일시적인 단백질 결합 확인 가능
Save precious antibodies
-
Reliable supply and quality
Consistency in staining
Reproducibility
-
Straightforward protocol and workflow
No picture enhancement needed
기존의 immunofluorescense 이미징 장비를 이용한 시각화
Features
-
Investigate unmodified cells and tissue
Detect proteins in their native state, without any overexpression or modifications.
-
Spatial (contextual) analysis
Retain protein localization : Visualize proteins as they are
-
High specificity
Two antibodies detect two different epitopes on the same protein or complex
-
Excellent signal resolution
Obtain clear target detection
-
Digitally quantify data
Works with several image analysis software’s- dot counting
-
Assay within one day
6 hour protocol NaveniFlex assay
-
High-quality reagents
Navenibodies : carefully selected antibodies conjugated to proprietary oligo arms
-
High sensitivity
NaveniFlex unique probe design significantly enhances the signal
Clear target detection
단백질의 과발현없이 현미경으로 직접 발현확인 Higher Signal to Noise ratio : Reduced tissue background
Sensitive in situ detection
Endogenous 상태에서 발현량이 약하거나 일시적인 단백질 결합 확인 가능 Save precious antibodies
Reliable supply and quality
Consistency in staining Reproducibility
Straightforward protocol and workflow
No picture enhancement needed 기존의 immunofluorescense 이미징 장비를 이용한 시각화
-
Investigate unmodified cells and tissue
Detect proteins in their native state, without any overexpression or modifications.
-
Spatial (contextual) analysis
Retain protein localization : Visualize proteins as they are
-
High specificity
Two antibodies detect two different epitopes on the same protein or complex
-
Excellent signal resolution
Obtain clear target detection
-
Digitally quantify data
Works with several image analysis software’s- dot counting
-
Assay within one day
6 hour protocol NaveniFlex assay
-
High-quality reagents
Navenibodies : carefully selected antibodies conjugated to proprietary oligo arms
-
High sensitivity
NaveniFlex unique probe design significantly enhances the signal
Applications
* Possibility to detect low abundant proteins
* Specific detection of post-translational modifications, also with the use of pan-specific antibodies
* Detection of protein-protein interactions
Protein detection PDL1
Cells HDLM-2, SignalSlide® PD-L1 IHC Controls 13747
Detection fluorophore TEX615
NaveniFlex MM
Detection of phosphorylation of PDGFR-ß
Cells fresh frozen, PFA fixed BJhTert cells
Detection fluorophore TEX615
NaveniFlex MR
Protein interaction of proximity between H3K4me3 & H3K27me3
Cells Neuronal Stem/Precursor Cells (primof proximity between H3K4me3 & H3K27me3 ary cultures)
Detection fluorophore Atto647N
NaveniFlex MR
Protein detection beta catenin and E-Cadherin
Tissue FFPE Skin Tissue
Detection fluorophore TEX615
NaveniFlex GR
Protein interaction of beta-catenin and e-cadherin
Tissue Human skin tissue
Detection fluorophore ATTO488
NaveniFlex MR
Protein interaction between CD8a & HLAa
Tissue Lung cancer FFPE tissue
Detection fluorophore ATTO488
NaveniFlex MR
Research Area
-
Immuno-profiling -
Treatment validation -
Signaling profiling -
Biomarker discovery -
Drug testing -
Diagnostics